The Musa Genotyping Centre (MGC) was established at the Laboratory of Molecular Cytogenetics and Cytometry of the Institute of Experimental Botany (IEB), Olomouc, Czech Republic in 2009. A standardized platform for molecular characterization has been developed for Musa germplasm (Christelová et al. 2011). The system is based on 19 microsatellite loci that are scored after the PCR with fluorescently labeled primers and capillary electrophoretic separation with internal standard (GeneScanTM-500 LIZ size standard, Applied Biosystems). The PCR products are multiplexed prior to the separation and loaded onto the automatic 96-capillary ABI 3730xl DNA Analyzer. Electrophoretic separation and signal detection is carried out with default module settings. The resulting data are then analyzed and called for alleles using GeneMarker® v1.75 (Softgenetics).
The Musa Genotyping Centre (MGC) in the Czech Republic conducts SSR genotyping and ploidy determination for the Musa community. Anyone can send leaf samples to MGC following these detailed instructions. Please contact Jaroslav Dolezel before sending your samples
The MGC operates at a cost-recovery basis and senders are expected to cover the cost of shipment of their samples to MGC.